PA5-27696

antibody from Invitrogen Antibodies

Targeting: BAG3

Antibody data

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Validation data

Product number: PA5-27696 - Provider product page
Provider: Invitrogen Antibodies
Product name: BAG3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: K562, mouse heart. Predicted reactivity: Mouse (94%), Rat (95%), Rhesus Monkey (100%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.41 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

BAG3 protein structure - PA5-27696 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BAG3 using 30 µg K562 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BAG3 polyclonal antibody (Product # PA5-27696) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-BAG3 Polyclonal Antibody (Product # PA5-27696) and a 74 kDa band corresponding to BAG family molecular chaperone regulator 3 was observed across the first three cell lines. Whole cell extracts (30 µg lysate) of HT-1080 (Lane 1), U-87 MG (Lane 2), DU 145 (Lane 3), SW480 (Lane 4), HEL 92.1.7 (Lane 5), K-562 (Lane 6), Jurkat (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using BAG3 Polyclonal Antibody (Product # PA5-27696). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 Polyclonal Antibody (Product # PA5-27696) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BAG3 Polyclonal Antibody detects BAG3 protein by western blot analysis. A. 50 µg mouse heart lysate/extract.7.5% SDS-PAGE. BAG3 Polyclonal Antibody (Product # PA5-27696) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.