HPA019652

antibody from Atlas Antibodies

Targeting: POU2F3 Epoc-1, OCT11, PLA-1, Skn-1a

Provider product page for HPA019652

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: HPA019652 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA019652, RRID:AB_1855585
Product name: Anti-POU2F3
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human POU2F3, Gene description: POU class 2 homeobox 3, Alternative Gene Names: Epoc-1, OCT11, PLA-1, Skn-1a, Validated applications: WB, IHC, Uniprot ID: Q9UKI9, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

POU2F3 protein structure - HPA019652 shown in red.

Submitted references Tuft cells act as regenerative stem cells in the human intestine

Huang L, Bernink J, Giladi A, Krueger D, van Son G, Geurts M, Busslinger G, Lin L, Begthel H, Zandvliet M, Buskens C, Bemelman W, López-Iglesias C, Peters P, Clevers H
Nature 2024;634(8035):929-935

Characterization of two transcriptomic subtypes of marker-null large cell carcinoma of the lung suggests different origin and potential new therapeutic perspectives

Simbolo M, Centonze G, Gkountakos A, Monti V, Maisonneuve P, Golovco S, Sabella G, Del Gobbo A, Gobbo S, Ferrero S, Fabbri A, Pardo C, Garzone G, Prinzi N, Pusceddu S, Testi A, Rolli L, Mangogna A, Bercich L, Benvenuti M, Bria E, Pilotto S, Berruti A, Pastorino U, Capella C, Infante M, Milella M, Scarpa A, Milione M
Virchows Archiv 2024;484(5):777-788

Ireland A, Hawgood S, Xie D, Barbier M, Lucas-Randolph S, Tyson D, Zuo L, Witt B, Govindan R, Dowlati A, Moser J, Puri S, Rudin C, Chan J, Elliott A, Oliver T
2024

EZH2 and POU2F3 Can Aid in the Distinction of Thymic Carcinoma from Thymoma.

Naso JR, Vrana JA, Koepplin JW, Molina JR, Roden AC
Cancers 2023 Apr 13;15(8)

OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage

Wu X, He X, Ipsaro J, Huang Y, Preall J, Ng D, Shue Y, Sage J, Egeblad M, Joshua-Tor L, Vakoc C
Nature 2022;607(7917):169-175

Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair

Gentile M, Barr J, Lee S, Kotas M, Fernanda de Mello Costa M, Holcomb N, Jaquish A, Palashikar G, Soewignjo M, McDaniel M, Matsumoto I, Margolskee R, Von Moltke J, Cohen N, Sun X, Vaughan A
eLife 2022;11

Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells

Huang H, Fang Y, Jiang M, Zhang Y, Biermann J, Melms J, Danielsson J, Yang Y, Qiang L, Liu J, Zhou Y, Wang M, Hu Z, Wang T, Saqi A, Sun J, Matsumoto I, Cardoso W, Emala C, Zhu J, Izar B, Mou H, Que J
eLife 2022;11

ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer

Olsen R, Ireland A, Kastner D, Groves S, Spainhower K, Pozo K, Kelenis D, Whitney C, Guthrie M, Wait S, Soltero D, Witt B, Quaranta V, Johnson J, Oliver T
Genes & Development 2021;35(11-12):847-869

Tuft Cells Increase Following Ovine Intestinal Parasite Infections and Define Evolutionarily Conserved and Divergent Responses

Hildersley K, McNeilly T, Gillan V, Otto T, Löser S, Gerbe F, Jay P, Maizels R, Devaney E, Britton C
Frontiers in Immunology 2021;12

Rlf–Mycl Gene Fusion Drives Tumorigenesis and Metastasis in a Mouse Model of Small Cell Lung Cancer

Ciampricotti M, Karakousi T, Richards A, Quintanal-Villalonga À, Karatza A, Caeser R, Costa E, Allaj V, Manoj P, Spainhower K, Kombak F, Sanchez-Rivera F, Jaspers J, Zavitsanou A, Maddalo D, Ventura A, Rideout W, Akama-Garren E, Jacks T, Donoghue M, Sen T, Oliver T, Poirier J, Papagiannakopoulos T, Rudin C
Cancer Discovery 2021;11(12):3214-3229

An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma

Zewdu R, Mehrabad E, Ingram K, Fang P, Gillis K, Camolotto S, Orstad G, Jones A, Mendoza M, Spike B, Snyder E
eLife 2021;10

MYC Drives Temporal Evolution of Small Cell Lung Cancer Subtypes by Reprogramming Neuroendocrine Fate

Ireland A, Micinski A, Kastner D, Guo B, Wait S, Spainhower K, Conley C, Chen O, Guthrie M, Soltero D, Qiao Y, Huang X, Tarapcsák S, Devarakonda S, Chalishazar M, Gertz J, Moser J, Marth G, Puri S, Witt B, Spike B, Oliver T
Cancer Cell 2020;38(1):60-78.e12

POU2F3 is a master regulator of a tuft cell-like variant of small cell lung cancer

Huang Y, Klingbeil O, He X, Wu X, Arun G, Lu B, Somerville T, Milazzo J, Wilkinson J, Demerdash O, Spector D, Egeblad M, Shi J, Vakoc C
Genes & Development 2018;32(13-14):915-928

Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites

Gerbe F, Sidot E, Smyth D, Ohmoto M, Matsumoto I, Dardalhon V, Cesses P, Garnier L, Pouzolles M, Brulin B, Bruschi M, Harcus Y, Zimmermann V, Taylor N, Maizels R, Jay P
Nature 2016;529(7585):226-230

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and POU2F3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415336).
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA019652 antibody. Corresponding POU2F3 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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