LS-C829079

antibody from LSBio

Targeting: POU2F3 Epoc-1, OCT11, PLA-1, Skn-1a

Antibody data

Submit

Validation data

Product number: LS-C829079 - Provider product page
Provider: LSBio
Product name: POU2F3 / PLA-1 Antibody (clone 34H8) LS-C829079
Antibody type: Monoclonal
Description: Protein A purified
Reactivity: Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 34H8
Storage: Store lyophilized at -20°C for up to 1 year. Store reconstituted at 2°C to 8°C for up to 3 weeks. Aliquot and store at -20°C or below for up to 1 year. Avoid freeze/thaw cycles.

POU2F3 protein structure - LS-C829079 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Western Blot of Sp2/0 cell lysates in different concentration with two independent antibodies: Mouse Epoc-1 Antibody (34H8) and Mouse Epoc-1 Antibody (39F11). The correlated pattern indicates the high specificity of these two antibodies. The different concentration of cell lysates indicates the high affinity and sensitivity of the antibodies. Lane 1: 100 µg Sp2/0 cell lysate. Lane 2: 50 µg Sp2/0 cell lysate. Lane 3: 25 µg Sp2/0 cell lysate. Lane 4: 50 ng recombinant mouse Epoc-1 protein. Lane 5: 100 µg Sp2/0 cell lysate. Lane 6: 50 µg Sp2/0 cell lysate. Lane 7: 25 µg Sp2/0 cell lysate. Lane 8: 50 ng recombinant mouse Epoc-1 protein. Primary Antibody: Lane 1~4: Mouse Epoc-1 Antibody (34H8) 1 µg/ml. Lane 5~8: Mouse Epoc-1 Antibody (39F11) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Western Blot of Sp2/0 cell lysates in different concentration with two independent antibodies: Mouse Epoc-1 Antibody (34H8) and Mouse Epoc-1 Antibody (39F11). The correlated pattern indicates the high specificity of these two antibodies. The different concentration of cell lysates indicates the high affinity and sensitivity of the antibodies. Lane 1: 100 µg Sp2/0 cell lysate. Lane 2: 50 µg Sp2/0 cell lysate. Lane 3: 25 µg Sp2/0 cell lysate. Lane 4: 50 ng recombinant mouse Epoc-1 protein. Lane 5: 100 µg Sp2/0 cell lysate. Lane 6: 50 µg Sp2/0 cell lysate. Lane 7: 25 µg Sp2/0 cell lysate. Lane 8: 50 ng recombinant mouse Epoc-1 protein. Primary Antibody: Lane 1~4: Mouse Epoc-1 Antibody (34H8) 1 µg/ml. Lane 5~8: Mouse Epoc-1 Antibody (39F11) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Standard curve of Epoc-1 Sandwich ELISA. The Epoc-1 Sandwich ELISA assay is developed by using Mouse Epoc-1 Antibody (34H8) and Mouse Epoc-1 Antibody (39F11) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, Mouse Epoc-1 Antibody (39F11) was labeled with Biotin. The sensitivity is < 1 ng/ml and the detection range is 0-100 ng/ml.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: ELISA binding of Mouse Epoc-1 Antibody (34H8) with recombinant Mouse Epoc-1 protein. Coating antigen: Epoc-1, 1 ug/ml. Epoc-1 antibody dilution start from 1000 ng/ml, EC50= 23.12 ng/ml.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: ELISA binding of Mouse Epoc-1 Antibody (34H8) with recombinant Mouse Epoc-1 protein. Coating antigen: Epoc-1, 1 ug/ml. Epoc-1 antibody dilution start from 1000 ng/ml, EC50= 23.12 ng/ml.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Standard curve of Epoc-1 Sandwich ELISA. The Epoc-1 Sandwich ELISA assay is developed by using Mouse Epoc-1 Antibody (34H8) and Mouse Epoc-1 Antibody (39F11) as capture and detection antibody, respectively. These two antibodies recognize different epitopes. In this ELISA assay, Mouse Epoc-1 Antibody (39F11) was labeled with Biotin. The sensitivity is < 1 ng/ml and the detection range is 0-100 ng/ml.