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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- NBP1-83966 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83966, RRID:AB_11024500
- Product name
- Rabbit Polyclonal POU2F3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human POU2F3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU2F3 Antibody [NBP1-83966] - Analysis in control (vector only transfected HEK293T lysate) and POU2F3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415336).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU2F3 Antibody [NBP1-83966] - Analysis in control (vector only transfected HEK293T lysate) and POU2F3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human skeletal muscle shows no positivity in myocytes
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Analysis in human skin and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human kidney shows no positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human pancreas shows no positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: POU2F3 Antibody [NBP1-83966] - Analysis of human airway cell line using POU2F3 antibody. Nuclear staining with DAPI. Image overlap in right panel. IHC-Fr image submitted by a verified customer review.
