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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-77836 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSPB8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunohistochemistry samples may be prepared using Bouin's Fixative solution. A 1:1,000 dilution of PA5-77836 was sufficient for detection of HSP22 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 22kDa. Does not cross-react with HSP27 or alpha-crystallin.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPB8 in rat skeletal muscle lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with HSPB8 polyclonal antibody (Product # PA5-77789) using a dilution of 1:1000 (2 hours at 4°C), followed by Donkey Anti-Rabbit HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HSPB8 in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with HSPB8 polyclonal antibody (Product # PA5-77789) using a dilution of 1:100 (12 hours at 4°C), and followed by FITC Goat Anti-Rabbit secondary antibody at a dilution of 1:50 (1-2 hours at RT). Magnification: 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPB8 in mouse back skin. Sample was fixed using Bouin's fixative solution, incubated with HSPB8 polyclonal antibody (Product # PA5-77789) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPB8 in mouse spinal cord. Sample was incubated with HSPB8 polyclonal antibody (Product # PA5-77789) using a dilution of 1:100, and followed by Alexa Fluor 488 Goat Anti-Rabbit and DAPI (blue).
