GTX79388

antibody from GeneTex

Targeting: PLAU UPA, URK

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX79388

Provider

GeneTex

Proper citation

GeneTex Cat#GTX79388, RRID:AB_11167093

Product name

uPA antibody (FITC)

Antibody type

Polyclonal

Host

Sheep

Vial size

1 mg

References

Submitted references

T-Cell Prolymphocytic Leukemia, Small Cell Variant, Possibly at the Stage of Intracytoplasmic Expression of CD3 in T-Cell Ontogenesis.

Yoshioka Y, Nagao M, Saitoh T, Yoshioka S, Tsunemine H, Itoh K, Kodaka T, Takahashi T
Journal of clinical and experimental hematopathology : JCEH 2015;55(1):17-21

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of TCR beta F1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with TCR Beta F1 antibody [8A3] at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10*6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR Beta F1 monoclonal antibody (GTX79388) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis

Validation comment

FACS