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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB9185-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse u-Plasminogen Activator (uPA)/Urokinase Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse uPA in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P06869- Isotype
- IgG
- Antibody clone number
- 901420
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of u-Plasminogen Activator (uPA)/Urokinase by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- u-Plasminogen Activator (uPA)/Urokinase in Mouse Kidney. u-Plasminogen Activator (uPA)/Urokinase was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
