sc-732

antibody from Santa Cruz Biotechnology

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-732

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-732, RRID:AB_2218221

Product name

Anti-YWHAQ

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.

Okamoto Y, Shirakashi Y, Ihara M, Urushitani M, Oono M, Kawamoto Y, Yamashita H, Shimohama S, Kato S, Hirano A, Tomimoto H, Ito H, Takahashi R
PloS one 2011;6(5):e20427

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Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms.

Rajagopalan S, Sade RS, Townsley FM, Fersht AR
Nucleic acids research 2010 Jan;38(3):893-906

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Novel snail1 target proteins in human colon cancer identified by proteomic analysis.

Larriba MJ, Casado-Vela J, Pendás-Franco N, Peña R, García de Herreros A, Berciano MT, Lafarga M, Casal JI, Muñoz A
PloS one 2010 Apr 20;5(4):e10221

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14-3-3 and its binding partners are regulators of protein-protein interactions during spermatogenesis.

Sun S, Wong EW, Li MW, Lee WM, Cheng CY
The Journal of endocrinology 2009 Sep;202(3):327-36

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Inhibition of PI-3K restores nuclear p27Kip1 expression in a mouse model of Kras-driven lung cancer.

Kelly-Spratt KS, Philipp-Staheli J, Gurley KE, Hoon-Kim K, Knoblaugh S, Kemp CJ
Oncogene 2009 Oct 15;28(41):3652-62

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ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery.

Jung CH, Jun CB, Ro SH, Kim YM, Otto NM, Cao J, Kundu M, Kim DH
Molecular biology of the cell 2009 Apr;20(7):1992-2003

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Presence and distribution of 14-3-3 proteins in human ocular surface tissues.

Shankardas J, Senchyna M, Dimitrijevich SD
Molecular vision 2008;14:2604-15

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14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr.

Bolton DL, Barnitz RA, Sakai K, Lenardo MJ
Biology direct 2008 Apr 29;3:17

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Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40

Emilie Vander Haar, Seong-il Lee, Sricharan Bandhakavi, Timothy J. Griffin, Do-Hyung Kim
Nature Cell Biology 2007 Feb;9(3):316-323

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The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival.

Lau JM, Jin X, Ren J, Avery J, DeBosch BJ, Treskov I, Lupu TS, Kovacs A, Weinheimer C, Muslin AJ
Molecular and cellular biology 2007 Feb;27(4):1455-66

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Molecular dynamics and in vitro analysis of Connexin43: A new 14-3-3 mode-1 interacting protein.

Park DJ, Freitas TA, Wallick CJ, Guyette CV, Warn-Cramer BJ
Protein science : a publication of the Protein Society 2006 Oct;15(10):2344-55

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14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase.

Pozuelo Rubio M, Peggie M, Wong BH, Morrice N, MacKintosh C
The EMBO journal 2003 Jul 15;22(14):3514-23

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 28

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.