LS-C359423

antibody from LSBio

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C359423

Provider

LSBio

Product name

YWHAQ / 14-3-3 Theta Antibody (aa125-175) LS-C359423

Antibody type

Polyclonal

Description

Protein A purified

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Feline, Hamster, Porcine, Simian

Host

Rabbit

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: 14-3-3 Antibody - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: 14-3-3 Antibody - Western blot shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.