Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C359466 - Provider product page
- Provider
- LSBio
- Product name
- YWHAQ / 14-3-3 Theta Antibody (aa40-90) LS-C359466
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Simian
- Host
- Rabbit
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot: 14-3-3 Antibody - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot: 14-3-3 Antibody - Western blot shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.