Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-86352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 theta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 theta in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: C6 whole cell lysate (40 µg), Lane 3: NIH-3T3 whole cell lysate (40 µg). Sample was incubated with 14-3-3 theta polyclonal antibody (Product # PA5-86352) using a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 14-3-3 theta in A375 cells. Sample was incubated with polyclonal 14-3-3 theta antibody (Product # PA5-86352) using a 1:100 dilution, and followed by goat anti-mouse IgG (H+L)-FITC and Hoechst (nuclei staining) secondary antibodies at a dilution of 1:400.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 14-3-3 theta in paraffin-embedded human kidney carcinoma tissue showing cytoplasmic/nucleus staining and negative control (right). Sample was incubated with polyclonal 14-3-3 theta antibody (Product # PA5-86352) using a 1:50 dilution, and followed by goat anti-rabbit IgG-biotin and avidin-peroxidase secondary antibodies.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 14-3-3 theta in paraffin-embedded human breast carcinoma tissue showing cytoplasm/nuclear staining and negative control (right). Sample was incubated with polyclonal 14-3-3 theta antibody (Product # PA5-86352) using a 1:50 dilution, and followed by goat anti-rabbit IgG-biotin and avidin-peroxidase secondary antibodies.