LS-B8314

antibody from LSBio

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B8314

Provider

LSBio

Product name

IHC-plus™ BLIMP1 / PRDM1 Antibody (aa698-711) LS-B8314

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Rabbit, Simian

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PRDM1 antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BLIMP1 / PRDM1 antibody (0.5µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PRDM1 / MEL1 Antibody (0.1µg/ml) staining of A431 (A), (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.