AF3608

antibody from Novus Biologicals

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3608

Provider

Novus Biologicals

Product name

Goat Polyclonal BLIMP1/PRDM1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and mouse BLIMP1/PRDM1 in direct ELISAs and Western blots.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

A role for Lin28 in primordial germ-cell development and germ-cell malignancy.

West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ
Nature 2009 Aug 13;460(7257):909-13

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BLIMP1/PRDM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3608) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BLIMP1/PRDM1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.