Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB36081 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal BLIMP1/PRDM1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BLIMP1/PRDM1 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation.
Effect of plasma-activated medium on the decrease of tumorigenic population in lymphoma.
IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM.
Chen D, Liu W, Zimmerman J, Pastor WA, Kim R, Hosohama L, Ho J, Aslanyan M, Gell JJ, Jacobsen SE, Clark AT
Cell reports 2018 Dec 26;25(13):3591-3602.e5
Cell reports 2018 Dec 26;25(13):3591-3602.e5
Effect of plasma-activated medium on the decrease of tumorigenic population in lymphoma.
Wada N, Ikeda JI, Tanaka H, Sakakita H, Hori M, Ikehara Y, Morii E
Pathology, research and practice 2017 Jul;213(7):773-777
Pathology, research and practice 2017 Jul;213(7):773-777
IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM.
Li S, Pal R, Monaghan SA, Schafer P, Ouyang H, Mapara M, Galson DL, Lentzsch S
Blood 2011 May 12;117(19):5157-65
Blood 2011 May 12;117(19):5157-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BLIMP1/PRDM1 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human BLIMP1/PRDM1 by Simple WesternTM. Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.