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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- IC36081R-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BLIMP1/PRDM1 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
O75626- Isotype
- IgG
- Antibody clone number
- 646702
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BLIMP1/PRDM1 in Human PBMCs gated on CD19+ CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+ CD3- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC36081R) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human CD38 PE-conjugated Monoclonal Antibody (Catalog # FAB2404P) and either (A) Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC36081R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
