Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB16909 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16909, RRID:AB_10678173
- Product name
- PRDM1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRDM1 .
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation.
Kinetics of a gamma interferon response: expression and assembly of CIITA promoter IV and inhibition by methylation.
Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells.
Fujita N, Jaye DL, Geigerman C, Akyildiz A, Mooney MR, Boss JM, Wade PA
Cell 2004 Oct 1;119(1):75-86
Cell 2004 Oct 1;119(1):75-86
Kinetics of a gamma interferon response: expression and assembly of CIITA promoter IV and inhibition by methylation.
Morris AC, Beresford GW, Mooney MR, Boss JM
Molecular and cellular biology 2002 Jul;22(13):4781-91
Molecular and cellular biology 2002 Jul;22(13):4781-91
Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells.
Nagy M, Chapuis B, Matthes T
British journal of haematology 2002 Feb;116(2):429-35
British journal of haematology 2002 Feb;116(2):429-35
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blots using PRDM1 polyclonal antibody (Cat # PAB16909) show detection of overexpressed PRDM1 in whole transfected Raji cell lysate (Lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1 (Lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (Lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1 : 1000 dilution in 5% PBS-Tween.Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.
