ABIN499475

antibody from antibodies-online

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN499475

Provider

antibodies-online

Product name

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (C-Term) antibody

Antibody type

Polyclonal

Antigen

Blimp-1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Blimp-1. (AP30154°CP-N)

Description

Affinity chromatography purified via peptide column

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

C-Term

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/mL

Storage

Store the antibody (in aliquots) at -20°C.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Transcriptional repressor Blimp-1 regulates T cell homeostasis and function.

Martins GA, Cimmino L, Shapiro-Shelef M, Szabolcs M, Herron A, Magnusdottir E, Calame K
Nature immunology 2006 May;7(5):457-65

---

Plasma cell differentiation requires the transcription factor XBP-1.

Reimold AM, Iwakoshi NN, Manis J, Vallabhajosyula P, Szomolanyi-Tsuda E, Gravallese EM, Friend D, Grusby MJ, Alt F, Glimcher LH
Nature 2001 Jul 19;412(6844):300-7

---

Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo.

Angelin-Duclos C, Cattoretti G, Lin KI, Calame K
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5462-71

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of Blimp-1 in A549 cell lysate with AP30154PU-N Blimp-1 antibody at (A) 0.5 and (B) 1?g/ml.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunofluorescence of Blimp-1 in mouse lung tissue with AP30154PU-N Blimp-1 antibody at 20 ?g/ml.