MA5-14879

antibody from Invitrogen Antibodies

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14879

Provider

Invitrogen Antibodies

Product name

Blimp-1 Monoclonal Antibody (E.995.1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

E.995.1

Vial size

100 µL

Concentration

10 µg/mL

Storage

-20°C

References

Submitted references

Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem Cells.

Li B, Qiao C, Jin X, Chan HM
Environmental health perspectives 2021 Jul;129(7):77007

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Blimp-1 in extracts from Karpas 620 and SR cell lines using Blimp-1 monoclonal antibody (Product # MA5-14879).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-Blimp-1 Monoclonal Antibody (E.995.1) (Product # MA5-14879) and a 92 kDa band corresponding to PR domain zinc finger protein 1 was observed across cell lines and tissue tested; was downregulated in Daudi upon MG132 treatment and upregulated in MDA-MB-231 upon TGF beta1 treatment. Whole cell extracts (30 µg lysate) of Raji (Lane 1), A549 (Lane 2), Daudi (Lane 3), Daudi treated with MG132 (1 µMpl/L for 24 hrs) (Lane 4), Ramos (Lane 5), MDA-MB-231 (Lane 6), MDA-MB-231 treated with TGF beta1 (5 ng/mL for 24 hrs) (Lane 7), KARPAS 299 (Lane 8), Mouse Thymus (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 10. Expressions of selected cell lineage marker proteins, (A) OCT4, (B) NANOG, (C) SOX2, (D) GATA4, and (E) PRDM1 as measured using western blots, and (F) IL6ST as measured using ELISAs in hESCs after 6 d of exposure to 0, 10, 50, 200 nM MeHg. Data are presented as means +- SEMs with N = 3 for NANOG and IL6ST; N = 4 for OCT4, GATA4, and PRDM1; and N = 7 for SOX2. For western blots, each batch was first normalized to its sodium carbonate vehicle control ( 0 nM ) before comparison. Thus, there are no error bars in the 0 nM MeHg groups for (A-E). The small circle symbols represent individual values of each experiment. One-way ANOVA was used at each time point to compare between the different doses, and the dose response of the treatment effects was significant. Dunnett's post hoc test was then used to compare the treatments to the vehicle control. Significance (*) was set at p < 0.05 . The corresponding numeric data are provided in Table S8. Note: ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; GATA4, GATA binding protein 4; hESC, human embryonic stem cell; IL6ST, interleukin 6 cytokine family signal transducer; MeHg, methylmercury; NANOG, Nanog homeobox; OCT4, octamer-binding transcription factor 4 [also known as POU5F1 (POU domain, class 5, transcription factor 1)]; PRDM1, PR domain-containing protein 1; SEM, standard error of the mean; SOX2, SRY-box transcription factor 2. Figures 10A to 10E are a set of one western blot and one bar graph. The we