Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- MA5-14879 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Blimp-1 Monoclonal Antibody (E.995.1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- E.995.1
- Vial size
- 100 µL
- Concentration
- 10 µg/mL
- Storage
- -20°C
Submitted references Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem Cells.
Li B, Qiao C, Jin X, Chan HM
Environmental health perspectives 2021 Jul;129(7):77007
Environmental health perspectives 2021 Jul;129(7):77007
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Blimp-1 in extracts from Karpas 620 and SR cell lines using Blimp-1 monoclonal antibody (Product # MA5-14879).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Blimp-1 Monoclonal Antibody (E.995.1) (Product # MA5-14879) and a 92 kDa band corresponding to PR domain zinc finger protein 1 was observed across cell lines and tissue tested; was downregulated in Daudi upon MG132 treatment and upregulated in MDA-MB-231 upon TGF beta1 treatment. Whole cell extracts (30 µg lysate) of Raji (Lane 1), A549 (Lane 2), Daudi (Lane 3), Daudi treated with MG132 (1 µMpl/L for 24 hrs) (Lane 4), Ramos (Lane 5), MDA-MB-231 (Lane 6), MDA-MB-231 treated with TGF beta1 (5 ng/mL for 24 hrs) (Lane 7), KARPAS 299 (Lane 8), Mouse Thymus (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 10. Expressions of selected cell lineage marker proteins, (A) OCT4, (B) NANOG, (C) SOX2, (D) GATA4, and (E) PRDM1 as measured using western blots, and (F) IL6ST as measured using ELISAs in hESCs after 6 d of exposure to 0, 10, 50, 200 nM MeHg. Data are presented as means +- SEMs with N = 3 for NANOG and IL6ST; N = 4 for OCT4, GATA4, and PRDM1; and N = 7 for SOX2. For western blots, each batch was first normalized to its sodium carbonate vehicle control ( 0 nM ) before comparison. Thus, there are no error bars in the 0 nM MeHg groups for (A-E). The small circle symbols represent individual values of each experiment. One-way ANOVA was used at each time point to compare between the different doses, and the dose response of the treatment effects was significant. Dunnett's post hoc test was then used to compare the treatments to the vehicle control. Significance (*) was set at p < 0.05 . The corresponding numeric data are provided in Table S8. Note: ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; GATA4, GATA binding protein 4; hESC, human embryonic stem cell; IL6ST, interleukin 6 cytokine family signal transducer; MeHg, methylmercury; NANOG, Nanog homeobox; OCT4, octamer-binding transcription factor 4 [also known as POU5F1 (POU domain, class 5, transcription factor 1)]; PRDM1, PR domain-containing protein 1; SEM, standard error of the mean; SOX2, SRY-box transcription factor 2. Figures 10A to 10E are a set of one western blot and one bar graph. The we