PA5-19216

antibody from Invitrogen Antibodies

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19216

Provider

Invitrogen Antibodies

Product name

Blimp-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response.

Li X, Dong Z, Liu Y, Song W, Pu J, Jiang G, Wu Y, Liu L, Huang X
Frontiers in immunology 2021;12:744880

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Blimp-1 by a Blimp-1 monoclonal antibody (Product # PA5-19216) at a concentration of 0.5 µg/mL. A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of A431 cell lysate using Product # PA5-19216 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Blimp-1 in A431 cells using a polyclonal antibody (Product #PA5-19216). A431 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 DENV suppressed NLRP12 expression by up-regulating PRDM-1 expression in a manner dependent on the TBK1/IRF3 and NF-kappaB signaling pathways. (A, B) hMDMs were infected with DENV (MOI=4) for indicated time periods and PRDM1 expression was determined by qPCR and western blot. The gray value of PRDM1 protein bands was analyzed by Image J gel image analysis software, and the relative grayscale value was normalized to that of beta-actin. (C) hMDMs were treated with Poly ( I:C ) and harvested for PRDM1 expression analysis. (D) The siRNA knockdown of PRDM1 was performed in hMDMs and the mRNA level of PRDM1 was measured. (E) PRDM1-silenced hMDMs were infected with DENV for 48h (MOI=4) and NLRP12 expression was determined via qPCR. (F) dTHP1 cells were infected with DENV with an MOI of 4 for 72h. Immunofluorescence staining was performed for DENV E protein, NLRP12, PRDM1 and DAPI (Bar: 10mum). (G) dTHP1 cells were transiently transfected with the siRNAs against the PRDM1, and then PRDM1-Flag-expressing vectors were transfected into the cells. After DENV infection at a MOI of 4 for 72h, the expression levels of NLRP12 and PRDM1 were detected by western blot analysis. The gray value of PRDM1 protein and NLRP12 bands was analyzed by Image J gel image analysis software and the relative grayscale value was normalized to that of beta-actin. (H, I) hMDMs cells were pre-treated with the inhibitors for TBK1 (BX795) or NF-kappaB (JSH-23) and subjected to DENV infection at a MOI of 4 f