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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09421PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09421PU-S, RRID:AB_2035877
- Product name
- anti PRDM1 / BLIMP1
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.15 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blots using affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween.
