600-401-B22

antibody from Rockland Immunochemicals, Inc.

Targeting: SERPINB2 HsT1201, PAI2, PLANH2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-B22

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-B22, RRID:AB_2186177

Product name

Anti-Plasminogen Activator Inhibitor 2 (RABBIT) Antibody - 600-401-B22

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG (p/n 611-103-122) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca D’Agostino, Temple University, Philadelphia, PA

Validation comment

Western Blot