MA5-15842

antibody from Invitrogen Antibodies

Targeting: BPTF FAC1, FALZ, NURF301

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15842

Provider

Invitrogen Antibodies

Product name

FALZ Monoclonal Antibody (2F10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2F10

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BPTF using BPTF monoclonal antibody (Product # MA5-15842) in HEK293 (1) and BPTF (AA: 503-670) human IgG Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BDTF (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a BDTF monoclonal antibody (Product # MA5-15842) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306).