NBP2-42204

antibody from Novus Biologicals

Targeting: ATXN1 ATX1, D6S504E, SCA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-42204

Provider

Novus Biologicals

Product name

Mouse Monoclonal Ataxin 1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 85kDa. No cross-reactivity against phosphor S751-Ataxin-1.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ataxin 1 Antibody (S65-37) [NBP2-42204] - analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of 85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 85 kDa.