MA5-27666

antibody from Invitrogen Antibodies

Targeting: ATXN1 ATX1, D6S504E, SCA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27666

Provider

Invitrogen Antibodies

Product name

Ataxin 1 Monoclonal Antibody (S76-8)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S76-8

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Ataxin 1 in monkey COS-1 cells transfected with Ataxin- 1 with 15 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with Ataxin 1 monoclonal antibody (Product # MA5-27666) using a dilution of 1:200 (16 hours at RT), and followed by Goat Anti-Mouse (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder, Lane 2: Monkey COS-1 cells transfected with Ataxin- 1.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ataxin 1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Ataxin 1 monoclonal antibody (Product # MA5-27666) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Ataxin 1 Antibody, D) Merged image. Magnification: 60x.