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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-28734 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28734, RRID:AB_1913918
- Product name
- Rabbit Polyclonal Ataxin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Ataxin 1 Antibody [NBP1-28734] - Detection of Human Ataxin-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Ataxin-1 antibody NBP1-28734 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Ataxin-1 was also immunoprecipitated by rabbit anti-Ataxin-1 antibody NBP1-28690, which recognizes an upstream epitope. For blotting immunoprecipitated Ataxin-1, the ReliaBLOT (RESTRICTED) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
