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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX119336 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX119336, RRID:AB_10617484
- Product name
- Dab1 antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE GTX119336 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX119336 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dab1 antibody [N1N3] (GTX119336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DAB1 antibody [N1N3] detects DAB1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DAB1 protein stained by DAB1 antibody [N1N3] (GTX119336) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DAB1 antibody [N1N3] detects DAB1 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. DAB1 antibody [N1N3] (GTX119336) dilution: 1:500.
