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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-85453 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAB1 in A) mouse brain using DAB1 polyclonal antibody (Product # PA5-85453) using 50 µg of sample at a dilution of 1:3000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DAB1 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DAB1 Polyclonal Antibody (Product # PA5-85453) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DAB1 in 4% paraformaldehyde-fixed HepG2 cells using DAB1 polyclonal antibody (Product # PA5-85453) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DAB1 in paraffin-embedded mouse duodenum using DAB1 polyclonal antibody (Product # PA5-85453) at a dilution of 1:500.
