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antibody from Invitrogen Antibodies
Targeting: ACSL1
ACS1, FACL1, FACL2, LACS, LACS1, LACS2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-68573 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 79%; Horse: 85%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 79%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSL1 on human Hela cells. The sample was probed with a ACSL1 polyclonal antibody (Product # PA5-68573) using a primary antibody dilution of .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ACSL1 Polyclonal Antibody (Product # PA5-68573) and 77 kDa band corresponding to ACSL1 was observed in Mouse and Rat Adipose, Mouse and Rat Liver but not in Kidney and Spleen which are reported to be low expressing for ACSL1. Tissue extracts (30 µg lysate) of Mouse Adipose (Lane 1), Mouse Spleen (Lane 2), Mouse Kidney (Lane 3), Mouse Liver (Lane 4), Rat Adipose (Lane 5), Rat Spleen (Lane 6), Rat Kidney (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on heart, liver, pancreas and brain tissue using a ACSL1 polyclonal antibody (Product # PA5-68573).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on heart, liver, pancreas and brain tissue using a ACSL1 polyclonal antibody (Product # PA5-68573).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on heart, liver, pancreas and brain tissue using a ACSL1 polyclonal antibody (Product # PA5-68573).
