PA5-17136

antibody from Invitrogen Antibodies

Targeting: ACSL1 ACS1, FACL1, FACL2, LACS, LACS1, LACS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17136

Provider

Invitrogen Antibodies

Product name

ACSL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

36 µg/mL

Storage

-20°C

References

Submitted references

Proteomic and Structural Manifestations of Cardiomyopathy in Rat Models of Obesity and Weight Loss.

Liśkiewicz AD, Marczak Ł, Bogus K, Liśkiewicz D, Przybyła M, Lewin-Kowalik J
Frontiers in endocrinology 2021;12:568197

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Ca2+ Binding/Permeation via Calcium Channel, CaV1.1, Regulates the Intracellular Distribution of the Fatty Acid Transport Protein, CD36, and Fatty Acid Metabolism.

Georgiou DK, Dagnino-Acosta A, Lee CS, Griffin DM, Wang H, Lagor WR, Pautler RG, Dirksen RT, Hamilton SL
The Journal of biological chemistry 2015 Sep 25;290(39):23751-65

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACSL1 in extracts from HepG2 and LN18 cells using ACSL1 polyclonal antibody (Product # PA5-17136).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ACSL1 Polyclonal Antibody (Product # PA5-17136) and a 77 kDa band corresponding to ACSL1 was observed across cell lines and tissues tested except in MCF7, undifferentiated 3T3-L1 and Mouse Spleen, which are reported to be negative for ACSL1. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF-10A (Lane 2), 3T3-L1 (Lane 3), 3T3-L1 differentiated to adipocytes (Lane 4), tissue extracts of Mouse Adipose (Lane 5), Mouse Spleen (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Kidney (Lane 9) and Rat Adipose (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ACSL1 was performed using 70% confluent log phase MCF-10A and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ACSL1 Polyclonal Antibody (Product # PA5-17136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondria like staining of ACSL1 in MCF-10A and not in MCF7 (panel e) which is reported negative for the same. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Molecular processes changed in the heart muscle of obese rats (n=5). A scaling plot of individual proteomes revealed the relatively homogenous composition in the control group (ContO) and the more dispersed proteomic phenotype in experimental (obese) animals (A) .Two biological processes: (B) the directed movement of a phospholipid out of a cell or organelle (p=0.024), and (C) the chemical reactions and pathways involving ATP (p=0.043), were significantly changed in the tissue (Bonferoni correction). By using the INTACT database we determined that the pool of all significantly changed proteins interacted with GLUT-4 (27 proteins, p