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LearnPA5-22888
antibody from Invitrogen Antibodies
Targeting: ACSL1
ACS1, FACL1, FACL2, LACS, LACS1, LACS2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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- Product number
- PA5-22888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSL1 using a polyclonal antibody (Product # PA5-22888).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSL1 in HepG2 whole cell lysates. Sample was incubated in ACSL1 polyclonal antibody (Product # PA5-22888).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSL1 in 0.5 mg/mL HepG2 lysate. Samples were incubated in ACSL1 polyclonal (Product # PA5-22888). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ACSL1 Polyclonal Antibody (Product # PA5-22888) and a 77 kDa band corresponding to ACSL1 was observed in Mouse and Rat Adipose, Liver tissues but not in Kidney and Spleen which are reported to be low expressing for ACSL1. Tissue extracts (30 µg lysate) of Mouse Adipose (Lane 1), Mouse Spleen (Lane 2), Mouse Kidney (Lane 3), Mouse Liver (Lane 4), Rat Adipose (Lane 5), Rat Spleen (Lane 6), Rat Kidney (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 ug/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
