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LearnPA5-29506
antibody from Invitrogen Antibodies
Targeting: ACSL1
ACS1, FACL1, FACL2, LACS, LACS1, LACS2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji. Predicted reactivity: Pig (82%), Chimpanzee (99%), Bovine (84%), Guinea pig (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSL1 using 30 µg of A) Jurkat and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ACSL1 polyclonal antibody (Product # PA5-29506) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ACSL1 Polyclonal Antibody (Product # PA5-29506) and a 77 kDa band corresponding to ACSL1 was observed across the positive cell lines (Hep G2 and MCF-10A) and not in the negative model, MCF7 as reported. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), MCF7 (Lane 2) and MCF-10A (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). The blot was probed with the primary antibody (1:1200 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using ACSL1 (Product # PA5-29506) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
