Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-47816-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Caldesmon/CALD1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Electropherogram image of the corresponding Simple Western lane. Caldesmon/CALD1 antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Aorta lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Human Uterus stained with Caldesmon Monoclonal Antibody (h-CALD).