Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab092465 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Caldesmon/CDM Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Caldesmon/CDM Antibody, Recombinant, could be used for WB, IHC and so on.Application:WB: 1/500 Ð 1/1000IHC: 1/50-/100Protein FunctionActin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 782-793
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,2 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Caldesmon/CDM Antibody (Ab092465) - Western Blot All lanes: Recombinant Caldesmon/CDM Antibody (Ab092465) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 93 kDa Observed band size: 76 kDa Exposure time: 9.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Caldesmon/CDM Antibody (Ab092465) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Caldesmon/CDM Antibody (Ab092465). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.