Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-85701 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85701, RRID:AB_11054883
- Product name
- Rabbit Polyclonal Caldesmon/CALD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Caldesmon/CALD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection.
Profiling post-centrifugation delay of serum and plasma with antibody bead arrays.
Histochemical localization of caldesmon in the CNS and ganglia of the mouse.
Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Nov;61(11):773-84
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Nov;61(11):773-84
Profiling post-centrifugation delay of serum and plasma with antibody bead arrays.
Qundos U, Hong MG, Tybring G, Divers M, Odeberg J, Uhlen M, Nilsson P, Schwenk JM
Journal of proteomics 2013 Dec 16;95:46-54
Journal of proteomics 2013 Dec 16;95:46-54
Histochemical localization of caldesmon in the CNS and ganglia of the mouse.
Köhler CN
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 May;59(5):504-17
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 May;59(5):504-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caldesmon/CALD1 Antibody [NBP1-85701] - Western blot analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caldesmon/CALD1 Antibody [NBP1-85701] - Analysis using Anti-CALD1 antibody NBP1-85701 (A) shows similar pattern to independent antibody NBP1-85702 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human smooth muscle shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining in human smooth muscle and skeletal muscle tissues using anti-CALD1 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human cerebral cortex using Anti-CALD1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human placenta using Anti-CALD1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human cerebral cortex, placenta, skeletal muscle and smooth muscle using Anti-CALD1 antibody NBP1-85701 (A) shows similar protein distribution across tissues to independent antibody NBP1-85702 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human skeletal muscle using Anti-CALD1 antibody NBP1-85701.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human smooth muscle using Anti-CALD1 antibody NBP1-85701.