NBP1-85702

antibody from Novus Biologicals

Targeting: CALD1 CDM, H-CAD, L-CAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85702

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85702, RRID:AB_11039260

Product name

Rabbit Polyclonal Caldesmon/CALD1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Caldesmon/CALD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.

Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello DV, Byrom D, Cortina C, Morral C, Barceló C, Tosi S, Riera A, Attolini CS, Rossell D, Sancho E, Batlle E
Nature genetics 2015 Apr;47(4):320-9

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51

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Histochemical localization of caldesmon in the CNS and ganglia of the mouse.

Köhler CN
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 May;59(5):504-17

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Mapping the subcellular protein distribution in three human cell lines.

Fagerberg L, Stadler C, Skogs M, Hjelmare M, Jonasson K, Wiking M, Abergh A, Uhlén M, Lundberg E
Journal of proteome research 2011 Aug 5;10(8):3766-77

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis in human cell line U-138 MG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85702] - Analysis using Anti-CALD1 antibody NBP1-85702 (A) shows similar pattern to independent antibody NBP1-85701 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining in human smooth muscle and skeletal muscle tissues using anti-CALD1 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human cerebral cortex, placenta, skeletal muscle and smooth muscle using Anti-CALD1 antibody NBP1-85702 (A) shows similar protein distribution across tissues to independent antibody NBP1-85701 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human placenta.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Caldesmon/CALD1 Antibody [NBP1-85702] - Immunohistochemical staining of human smooth muscle shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human skeletal muscle using Anti-CALD1 antibody NBP1-85702.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human smooth muscle using Anti-CALD1 antibody NBP1-85702.