HPA008066

antibody from Atlas Antibodies

Targeting: CALD1 CDM, H-CAD, L-CAD

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA008066

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008066, RRID:AB_1078378

Product name

Anti-CALD1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human CALD1, Gene description: caldesmon 1, Alternative Gene Names: CDM, H-CAD, L-CAD, Validated applications: ICC, WB, IHC, Uniprot ID: Q05682, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Epithelial NOTCH Signaling Rewires the Tumor Microenvironment of Colorectal Cancer to Drive Poor-Prognosis Subtypes and Metastasis

Jackstadt R, van Hooff S, Leach J, Cortes-Lavaud X, Lohuis J, Ridgway R, Wouters V, Roper J, Kendall T, Roxburgh C, Horgan P, Nixon C, Nourse C, Gunzer M, Clark W, Hedley A, Yilmaz O, Rashid M, Bailey P, Biankin A, Campbell A, Adams D, Barry S, Steele C, Medema J, Sansom O
Cancer Cell 2019;36(3):319-336.e7

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TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis

Tauriello D, Palomo-Ponce S, Stork D, Berenguer-Llergo A, Badia-Ramentol J, Iglesias M, Sevillano M, Ibiza S, Cañellas A, Hernando-Momblona X, Byrom D, Matarin J, Calon A, Rivas E, Nebreda A, Riera A, Attolini C, Batlle E
Nature 2018;554(7693):538-543

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Stromal gene expression defines poor-prognosis subtypes in colorectal cancer

Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello D, Byrom D, Cortina C, Morral C, Barceló C, Tosi S, Riera A, Attolini C, Rossell D, Sancho E, Batlle E
Nature Genetics 2015;47(4):320-329

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Stromal gene expression defines poor-prognosis subtypes in colorectal cancer

Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello D, Byrom D, Cortina C, Morral C, Barceló C, Tosi S, Riera A, Attolini C, Rossell D, Sancho E, Batlle E
Nature Genetics 2015 February;47(4):320-329

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

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Mapping the Subcellular Protein Distribution in Three Human Cell Lines

Fagerberg L, Stadler C, Skogs M, Hjelmare M, Jonasson K, Wiking M, Åbergh A, Uhlén M, Lundberg E
Journal of Proteome Research 2011;10(8):3766-3777

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Histochemical localization of caldesmon in the CNS and ganglia of the mouse.

Köhler CN
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 May;59(5):504-17

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human prostate and skeletal muscle tissues using HPA008066 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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