MA5-44910

antibody from Invitrogen Antibodies

Targeting: WIPI1 ATG18, ATG18A, FLJ10055, WIPI49

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44910

Provider

Invitrogen Antibodies

Product name

WIPI1 Recombinant Rabbit Monoclonal Antibody (JE56-92)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE56-92

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WIPI1 in JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in WIPI1 Monoclonal antibody (Product # MA5-44910) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of WIPI1 in paraffin-embedded human fetal skeletal muscle tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with WIPI1 Monoclonal antibody (Product # MA5-44910) using a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.