Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-88878 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88878, RRID:AB_11028747
- Product name
- Rabbit Polyclonal WIPI1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human WIPI1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function.
Felzen V, Hiebel C, Koziollek-Drechsler I, Reißig S, Wolfrum U, Kögel D, Brandts C, Behl C, Morawe T
Cell death & disease 2015 Jul 9;6:e1812
Cell death & disease 2015 Jul 9;6:e1812
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIPI1 Antibody [NBP1-88878] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIPI1 Antibody [NBP1-88878] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIPI1 Antibody [NBP1-88878] - Analysis in human cell lines SK-MEL-30 and HeLa using anti-WIPI1 antibody. Corresponding WIPI1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human fallopian tube shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human placenta shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining in human placenta and fallopian tube tissues using anti-WIPI1 antibody. Corresponding WIPI1 RNA-seq data are presented for the same tissues.