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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-210-954-C050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-954-C050, RRID:AB_2052829
- Product name
- WIPI-1 (human) polyclonal antibody (AT129)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Epitope-affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis of human colorectal carcinoma cell lines HCT116 or RKO cells using PAb to WIPI-1 (human) (AT129)(Prod. No. ALX-210-954). Method: Total protein extracts from various human cell lines either untrasfected (lane 1), or transfected with a plasmid encoding human WIPI-I (lane 2) or with an empty plasmid (lane 3) were run on SDS-PAGE and WIPI-1 was detected by PAb to WIPI-1 (human) (AT129). Picture courtesy of Dr M.C. Maiuri, Guido Kroemer lab, Paris.
