Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AP7237A - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7237a, RRID:AB_2195815
- Product name
- SPHK1 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Sphingosine-1-phosphate and sphingosine kinase are critical for transforming growth factor-beta-stimulated collagen production by cardiac fibroblasts.
FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1.
Gellings Lowe N, Swaney JS, Moreno KM, Sabbadini RA
Cardiovascular research 2009 May 1;82(2):303-12
Cardiovascular research 2009 May 1;82(2):303-12
FHL-2 suppresses VEGF-induced phosphatidylinositol 3-kinase/Akt activation via interaction with sphingosine kinase-1.
Hayashi H, Nakagami H, Takami Y, Koriyama H, Mori M, Tamai K, Sun J, Nagao K, Morishita R, Kaneda Y
Arteriosclerosis, thrombosis, and vascular biology 2009 Jun;29(6):909-14
Arteriosclerosis, thrombosis, and vascular biology 2009 Jun;29(6):909-14
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "The anti-SphK1 Pab (Cat. #AP7237a) is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH)."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hSPHK1-M1 Pab (Cat. #AP7237a) in 293 cell line lysates transiently transfected with the SPHK1 gene (2ug/lane). hSPHK1-M1(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000