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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AP7237d - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7237d, RRID:AB_2195811
- Product name
- SPHK1 Antibody (N-term P74)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury.
A novel function of sphingosine kinase 1 suppression of JNK activity in preventing inflammation and injury.
Kim M, Park SW, Kim M, D'Agati VD, Lee HT
Anesthesiology 2011 Feb;114(2):363-73
Anesthesiology 2011 Feb;114(2):363-73
A novel function of sphingosine kinase 1 suppression of JNK activity in preventing inflammation and injury.
Di A, Kawamura T, Gao XP, Tang H, Berdyshev E, Vogel SM, Zhao YY, Sharma T, Bachmaier K, Xu J, Malik AB
The Journal of biological chemistry 2010 May 21;285(21):15848-57
The Journal of biological chemistry 2010 May 21;285(21):15848-57
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Supportive validation
- Submitted by
- Abgent (provider)
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- Experimental details
- "Western blot analysis of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate(from left to right), using SPHK1 Antibody (N-term P74)(Cat. #AP7237d). AP7237d was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
