TA309876

antibody from OriGene

Targeting: SPHK1 SPHK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA309876

Provider

OriGene

Product name

Rabbit Polyclonal Sphingosine Kinase 1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Sphingosine Kinase 1 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SPHK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.0mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody, which recognizes an upstream epitope

Validation comment

WB