Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F50217 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SPHK1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 286-315 from the human protein was used as the immunogen for this SPHK1 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the SPHK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) 293, 2) HepG2, 3) HUVEC, 4) Raji, 5) rat C6 and 6) mouse C2C12 cell line using SPHK1 antibody at 1:1000. Predicted molecular weight: 42-51 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The SPHK1 antibody used in western blot to detect SPHK1 in mouse kidney tissue lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of SPHK1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPHK1 gene.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the SPHK1 antibody