Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-14068 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPHK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Blocking SphK1/S1P/S1PR1 Signaling Pathway Alleviates Lung Injury Caused by Sepsis in Acute Ethanol Intoxication Mice.
Chen L, Li L, Song Y, Lv T
Inflammation 2021 Dec;44(6):2170-2179
Inflammation 2021 Dec;44(6):2170-2179
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPHK1 using a SPHK1 polyclonal antibody (Product # PA5-14068) in mouse kidney tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SPHK1 polyclonal antibody (Product # PA5-14068) in 293 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPHK1 (arrow) using a SPHK1 polyclonal antibody (Product # PA5-14068) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a SPHK1 polyclonal antibody (Product # PA5-14068), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 PF-543 blocks the SphK1/S1P/S1PR1 signaling pathway. The SphK1 activity ( A ) and S1P level ( B ) in lung tissues were detected by respective kit. C The protein expression of SphK1 and S1PR1 in lung tissues was determined by Western blot. D The mRNA expression of SphK1 and S1PR1 in lung tissues was determined by RT-qPCR analysis. *** P