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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-19477 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SPHK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-19477 targets SPHK1 in ICC, IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-19477 immunogen is synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human SPHK1. PA5-19477 detects SPHK1 which has a predicted molecular weight of approximately 69 kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.7 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Recombinant SPHK1 and GST tag using Product # PA5-19477, SPHK1 primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
