Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27705 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Jurkat.
- Concentration
- 0.76 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), KARPAS 299 (Lane 2), HEK-293 (Lane 3), Raji (Lane 4) and THP-1 (Lane 5). The blot was probed with Anti- TRAF5 Rabbit Polyclonal Antibody (Product # PA5-27705, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 64 kDa band corresponding to TRAF5 was detected across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRAF5 Polyclonal Antibody (Product # PA5-27705). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. TRAF5 Polyclonal Antibody (Product # PA5-27705) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRAF5 in methanol-fixed HeLa cells using a TRAF5 polyclonal antibody (Product # PA5-27705) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRAF5 Polyclonal Antibody detects TRAF5 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TRAF5 Polyclonal Antibody (Product # PA5-27705) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
