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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30386 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAIAP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: SK-N-SH, mouse brain, rat brain. Predicted reactivity: Mouse (98%), Rat (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAIAP2 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BAIAP2 polyclonal antibody (Product # PA5-30386) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAIAP2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BAIAP2 Polyclonal Antibody (Product # PA5-30386) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BAIAP2 Polyclonal Antibody (Product # PA5-30386). Sample (30 µg of whole cell lysate). Lane A: SK-N-SH. 7.5% SDS PAGE. BAIAP2 Polyclonal Antibody (Product # PA5-30386) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BAIAP2 in methanol-fixed A431 cells using a BAIAP2 polyclonal antibody (Product # PA5-30386) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BAIAP2 Polyclonal Antibody detects BAIAP2 protein at cytoplasm in Purkinje cells of mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BAIAP2 Polyclonal Antibody (Product # PA5-30386) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
