PA2140-2

antibody from Boster Biological Technology

Targeting: COL1A1 OI4

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA2140-2

Provider

Boster Biological Technology

Product name

Anti-Collagen I/COL1A1 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for COLLAGEN I/COL1A1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. COLLAGEN I/COL1A1 information: Molecular Weight: 138032 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons.

Sharma A, Hill KE, Schwarzbauer JE
Frontiers in cellular neuroscience 2023;17:1177663

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Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites.

Hill KE, Lovett BM, Schwarzbauer JE
The Journal of biological chemistry 2022 Jan;298(1):101479

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Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity.

Zhang P, The E, Luo Z, Zhai Y, Yao Q, Ao L, Fullerton DA, Xu D, Meng X
Molecular medicine (Cambridge, Mass.) 2022 Jan 21;28(1):5

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Efficacy of probiotic Streptococcus thermophilus in counteracting TGF-β1-induced fibrotic response in normal human dermal fibroblasts.

Lombardi F, Augello FR, Artone S, Bahiti B, Sheldon JM, Giuliani M, Cifone MG, Palumbo P, Cinque B
Journal of inflammation (London, England) 2022 Dec 19;19(1):27

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Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice.

Rodríguez-Escamilla JC, Medina-Reyes EI, Rodríguez-Ibarra C, Déciga-Alcaraz A, Flores-Flores JO, Ganem-Rondero A, Rodríguez-Sosa M, Terrazas LI, Delgado-Buenrostro NL, Chirino YI
Journal of applied toxicology : JAT 2019 Nov;39(11):1586-1605

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Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone.

Iskenderian A, Liu N, Deng Q, Huang Y, Shen C, Palmieri K, Crooker R, Lundberg D, Kastrapeli N, Pescatore B, Romashko A, Dumas J, Comeau R, Norton A, Pan J, Rong H, Derakhchan K, Ehmann DE
Skeletal muscle 2018 Oct 27;8(1):34

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Rat Lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image