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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- AF6220 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Collagen I alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Collagen I alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human COL3A1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule.
Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Vijayaraj P, Minasyan A, Durra A, Karumbayaram S, Mehrabi M, Aros CJ, Ahadome SD, Shia DW, Chung K, Sandlin JM, Darmawan KF, Bhatt KV, Manze CC, Paul MK, Wilkinson DC, Yan W, Clark AT, Rickabaugh TM, Wallace WD, Graeber TG, Damoiseaux R, Gomperts BN
Cell reports 2019 Dec 10;29(11):3488-3505.e9
Cell reports 2019 Dec 10;29(11):3488-3505.e9
Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
Bose C, Megyesi JK, Shah SV, Hiatt KM, Hall KA, Karaduta O, Swaminathan S
PloS one 2015;10(8):e0136563
PloS one 2015;10(8):e0136563
Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.
Mogami H, Keller PW, Shi H, Word RA
The Journal of biological chemistry 2014 May 9;289(19):13295-307
The Journal of biological chemistry 2014 May 9;289(19):13295-307
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Hewitt KJ, Shamis Y, Knight E, Smith A, Maione A, Alt-Holland A, Sheridan SD, Haggarty SJ, Garlick JA
Journal of cell science 2012 May 1;125(Pt 9):2276-87
Journal of cell science 2012 May 1;125(Pt 9):2276-87
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Collagen I alpha 1 by Western Blot. Western blot shows lysates of human kidney tissue, human skin tissue, and human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen I alpha 1 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
