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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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- Product number
- GTX112731 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112731, RRID:AB_10721155
- Product name
- COL1A1 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression.
No difference in renal injury and fibrosis between wild-type and NOD1/NOD2 double knockout mice with chronic kidney disease induced by ureteral obstruction.
Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
Conditional Deletion of Smad1 Ameliorates Glomerular Injury in Progressive Glomerulonephritis.
Chen HY, Huang TC, Lin LC, Shieh TM, Wu CH, Wang KL, Hong YH, Hsia SM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;49(5):1970-1986
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;49(5):1970-1986
No difference in renal injury and fibrosis between wild-type and NOD1/NOD2 double knockout mice with chronic kidney disease induced by ureteral obstruction.
Stroo I, Emal D, Butter LM, Teske GJ, Claessen N, Dessing MC, Girardin SE, Florquin S, Leemans JC
BMC nephrology 2018 Apr 2;19(1):78
BMC nephrology 2018 Apr 2;19(1):78
Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
Chen S, Peng C, Wei X, Luo D, Lin Y, Yang T, Jin X, Gong L, Li H, Wang K
World journal of urology 2017 Aug;35(8):1247-1254
World journal of urology 2017 Aug;35(8):1247-1254
Conditional Deletion of Smad1 Ameliorates Glomerular Injury in Progressive Glomerulonephritis.
Araki M, Matsubara T, Abe H, Torikoshi K, Mima A, Iehara N, Fukatsu A, Kita T, Arai H, Doi T
Scientific reports 2016 Aug 5;6:31216
Scientific reports 2016 Aug 5;6:31216
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE GTX112731 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE GTX112731 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody detects COL1A1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody detects COL1A1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody detects COL1A1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: COL1A1 protein stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: COL1A1 protein stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 £gg of COL1A1 antibody [N1N2], N-term (GTX112731).Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
